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K. Tan1, H.L. Zheng, S.P. Dong, M.X. Ren (2019)Molecular phylogeny of Hiptage (Malpighiaceae) reveals a new species from Southwest China. doi:10.5061/dryad.hqbzkh1bh

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Occurrence

South Cangshan mountain, Pingpo Town, Dali City

Pas de description disponible

Les personnes impliquées dans le projet:

The proposed new species was compared with the type specimens of all accepted names in the genus, including collections of Hiptage deposited in the herbaria KUN, PE, IBSC, and IBK (acronyms according to Thiers 2019). We also downloaded all Hiptage specimens from JSTOR Global Plants (http://plants.jstor.org), and Chinese Virtual Herbarium (http://www.cvh.ac.cn) to compare detailed morphological traits between the proposed new species with the currently accepted species of Hiptage.

Description des étapes de la méthode:

1. Total genomic DNA was extracted from dried leaf material following a modified CTAB method (Doyle and Doyle 1987). All polymerase chain reactions (PCR) were carried out in 25 μl volumes consisting of 1 μl sample DNA, 12.5 μl 2×Taq PCR master Mix (Aidlab Biotechnologies Co. Ltd), 1 μl each primer (10 μmol/ml), and a final volume adjusted to 25 μl with double distilled water. The ITS region was amplified with the primers ITS17SE and ITS26SE (Sun et al. 1994). We used an amplification profile with an initial denaturation of 5 min at 94°C, followed by 35 cycles of 40 seconds at 94°C, 20 seconds at 69°C, 1 min at 72°C, and a final 10 min extension at 72°C. The PCR products were sequenced from both directions using on an ABI3730XL sequencer.