Penile shape discriminates the two cryptic species of Akodon (Mammalia, Rodentia, Cricetidae) from eastern Brazil

Leonardo  Campana 1, Letícia Cruz2, Roberta  Paresque 3, Valéria  Fagundes4, Valeria Fagundes1, Valeria Fagundes5

1 Universidade Federal do Espírito Santo,  Av Fernando Ferrari, 514, Vitória, Brazil; 2 Universidade Federal do Espírito Santo ,  Av Fernando Ferrari, 514, Vitória , Brazil; 3 Universidade Federal do Espírito Santo, Rodovia BR 101 Norte, Km 60, São Mateus, Brazil; 4 Universidade Federal do Espírito Santo ,  Av Fernando Ferrari, 514, Vitória, Brazil; 5 Universidade Federal do Espírito Santo, Av Fernando Ferrari, 514, Vitória, Brazil

Corresponding author(s): Leonardo  Campana Letícia CruzRoberta  Paresque Valéria  Fagundes, Valeria Fagundes (valeria.fagundes@ufes.br)

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Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Abstract

The raw data contains the basis of Record, catalognumber, recordnumber, establishmentMeans (wild or captive born animals at Laboratory of Animal Genetics from Federal University of Espírito Santo, Brazil),  associatedorganisms (parentals of captive born animals, with municipality, stateprovince and diploid number), stateProvince, country, municipality, decimalLatitude, decimalLongitude and identificationRemarks (about diploid number) and scientificName. Wild specimens with UFES-MAM catalognumber are deposited in the Mammal Collection of Federal University of Espírito Santo and available in the online network database Species Link (https://specieslink.net/).

Keywords: Occurrence, Specimen

Project details

Project title: Penile shape discriminates the two cryptic species of Akodon (Mammalia, Rodentia, Cricetidae) from eastern Brazil
Personnel: Leonardo  Campana, Letícia Cruz, Roberta  Paresque, Valéria Fagundes
Funding: This work was funded by the Foundation for Research and Innovation of the state of Espírito - FAPES (grant #80600417/17 to VF) and the National Council for Scientific and Technological Development - CNPq (undergraduate bursary to LC and LRC and research bursary to VF). We thank the Laboratory of Cellular Ultrastructure Carlos Alberto Redins at UFES (LUCCAR-UFES) for assistance with Scanning Electron Microscopy. We are indebted to all field and the crossbreeding lab teams that, without them, this study would not be possible. We also thank Dr Wesley D. Colombo for assistance with photographing using the extended focus imaging system. In this study the collecting and processing specimens as well as access to the genetic heritage was duly registered with SisGen - Sistema Nacional de Gestão do Patrimônio Genético e dos Conhecimentos Tradicionais Associados, as required by Brazilian law. The authors have declared that no competing interests exist. 

Data published through GBIF: https://ipt.pensoft.net/resource?r=akodon-penile-shape

Spatial coverage

General spatial coverage: Wild caught and captive born specimens of Akodon cursor, Akodon montensis and your Hybrids
Coordinates: 25°2'45.6''S and 5°52'40.8''S Latitude; 53°52'37.2''W and 29°34'30''W Longitude 

Methods

Method step description: Firstly, six non-metric characters of the penile morphology were described to identify the character states. We focused on the following characters: spines morphology, dorsal cleft, dorsal cleft depth, ventral cleft, glans shape, and presence/absence of at least one dark spot on the ventral view of the glans (Table 2).
Then, using the software TPSDig2 Version 2.31 (Rohlf 2009), we took seven glans penis’ linear measurements of the ventral and dorsal given by photographic views, as follow: Total length (TL), i.e. the distance from the beginning of the dorsal base of the spined area to the distalmost point on the glans; Spined Area Length (SAL), i.e. the distance from the beginning of the dorsal base of the spined area to the distalmost point on the spined area; Base Width (BW) i.e. diameter of the glans on the beginning of the base of the spined area; Meddle Width (MW) i.e. the greatest diameter of the glans penis, usually on the middle part of the spined area; Tip Width (TW) i.e. diameter of the distal end of the spined area; Dorsal Cleft Width (DCL) i.e. distance from the distalmost point to the proximal-most point of the dorsal cleft; Medial Bacular Mound Width (MBMW) i.e. diameter of the base of the medial bacular mound (Figure 1b). To guarantee the reliability of the measuring methods, the measurements were taken five times by the same researcher.

Study extent description: Pernambuco, Bahia, Espírito Santo, Minas Gerais and São Paulo states, Brazil
Sampling description: We used 96 male adults (Table 1, Figure 1a, Supplementary data S1), being 14 wild types (11 A. cursor, and 3 A. montensis) and 82 captive-born (56 A. cursor, 27 A. montensis, and 13 hybrids). 
The penises were extracted by scissors, fixed in a 10% formalin solution for 24 hours and stored in a solution of 70% ethanol. Before analysis, they were air-dried at room temperature, and then ventral and dorsal views were photographed using an extended focus imaging system GT-Vision (©Leica microsystems). We used digital pictures with a scale bar to determine the linear measurements of each specimen.

Quality control description:  For captive-born grouping, we analysed individuals aged at least 3-month-old. The species identification and their hybrids was based on karyotype features: diploid number (2n), the number of autosomal arms or fundamental number (FN) and the morphology of autosomes.

Datasets

Dataset description
Object name: Darwin Core Archive Penile shape discriminates the two cryptic species of Akodon (Mammalia, Rodentia, Cricetidae) from eastern Brazil
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: https://ipt.pensoft.net/archive.do?r=akodon-penile-shape
Publication date of data: 2022-11-10
Language: English
Licences of use: Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License

Metadata language: English
Date of metadata creation: 2022-11-07
Hierarchy level: Dataset