croplm
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引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Kermek D, Pischiutta N, Hlebec D (2024): croplm. v1.2. Biodiversity Data Journal. Dataset/Occurrence. https://ipt.pensoft.net/resource?r=croplm&v=1.2
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は Biodiversity Data Journal。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: f5b16e64-610a-4fcf-b46d-3efb068caaa1が割り当てられています。 Participant Node Managers Committee によって承認されたデータ パブリッシャーとして GBIF に登録されているBiodiversity Data Journal が、このリソースをパブリッシュしました。
キーワード
Occurrence; Specimen; stoneflies; Medvednica Mt.; Croatia; biodiversity
連絡先
リソースを作成した人:
リソースに関する質問に答えることができる人:
メタデータを記載した人:
他に、リソースに関連付けられていた人:
地理的範囲
Croatia (Medvednica Mt.)
座標(緯度経度) | 南 西 [-90, -180], 北 東 [90, 180] |
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生物分類学的範囲
Stoneflies
Order | Plecoptera (Stoneflies) |
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時間的範囲
開始日 / 終了日 | 2021-06-17 / 2022-03-04 |
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プロジェクトデータ
Medvednica Nature Park, located near Croatia's capital and largest city, Zagreb, experiences significant urbanization, which strongly impacts the fauna within this ecosystem. Surprisingly, despite the area's diverse (micro)habitats, including the discovery of several rare species previously unknown in the Croatian fauna, comprehensive field research on any group of organisms, coupled with an integrative approach, has never been conducted in this region. In this study, we present the first insights into the genetic and morphological diversity of stoneflies, one of the most endangered groups of organisms overall. Phylogenetic reconstructions and species delineation methods revealed significant intraspecific haplotype variation in most examined species (e.g., Brachyptera seticornis, Isoperla grammatica, and Leuctra braueri), except for Leuctra prima. Additionally, our study has identified isolated populations that merit further in-depth investigation concerning morphology, genetics, and ecology. These findings contribute valuable knowledge to the conservation of stoneflies in this area.
タイトル | Utilizing public sequence databases to identify genetic diversity of stoneflies in Medvednica Nature Park |
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識別子 | Dora Kermek, Nikola Pischiutta |
ファンデイング | Croatian Science Foundation |
Study Area Description | Medvednica Mt., Croatia |
研究の意図、目的、背景など(デザイン) | Project provides the first insight into the genetic and morphological diversity of stoneflies in the Medvednica Nature Park, thereby contributing to the faunal knowledge of the area. Additionally, it establishes phylogenetic and phylogeographical relationships between populations of widely distributed species in Europe: Brachyptera seticornis, Isoperla grammatica, Leuctra braueri, and Leuctra prima. These relationships enhance understanding of diversification patterns and the biogeographic history of these selected stonefly species, emphasizing populations that warrant further detailed morphological, genetic, and ecological investigation. |
プロジェクトに携わる要員:
収集方法
Adults were collected using an entomological net while larvae were collected by hand under stones and branches.
Study Extent | Specimens were collected at eight sampling sites in Medvednica Nature Park. |
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Quality Control | All collected individuals were preserved in ethanol. Morphological determination of adults was conducted using a ZEISS SteREO Discovery V20 stereo microscope along with various identification keys. Due to the lack of identification keys the larvae were not determined to the species level. Larval identification was carried out by comparing their sequences with sequences available in databases. |
Method step description:
- Total genomic DNA was isolated from the tissues of one to three legs of adults and larvae using the GenElute Mammalian Genomic DNA Miniprep Kit. Chromatograms were analyzed and edited in Geneious Prime. Misread nucleotides were manually corrected. The presence of stop codons in all sequences was checked using Mesquite. Poor quality sequences and those containing stop codons were excluded from further analyses. For phylogeographic analyses, we selected species with a broader distribution and a larger number of COI sequences available in the BOLD and GenBank databases.
コレクションデータ
コレクション名 | Collection Plecoptera Sivec&Hlebec |
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標本保存方法 | Alcohol |
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Curatorialユニット | 範囲 488 ~ 512 vial |
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