croplm
最新版本 由 Biodiversity Data Journal 發佈於 2024年2月13日 Biodiversity Data Journal

Stoneflies of Medvednica Nature Park: genetic diversity and morphological variability

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Kermek D, Pischiutta N, Hlebec D (2024): croplm. v1.2. Biodiversity Data Journal. Dataset/Occurrence. https://ipt.pensoft.net/resource?r=croplm&v=1.2

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關鍵字

Occurrence; Specimen; stoneflies; Medvednica Mt.; Croatia; biodiversity

聯絡資訊

資源建立者:

Dora Kermek
Student
Faculty of Science, University of Zagreb
HR
Nikola Pischiutta
Student
Faculty of Science, University of Zagreb
Dora Hlebec
Assistant
Faculty of Science, University of Zagreb

可回覆此資源相關問題者:

Dora Hlebec
Assistant
Faculty of Science, University of Zagreb
Horvatovac 102a
10000 Zagreb
HR

元數據填寫者:

Dora Hlebec
Assistant
Faculty of Science, University of Zagreb
Horvatovac 102a
10000 Zagreb
HR

與此資源的相關者:

典藏經理
Iva Mihoci
Curator
Croatian Natural History Museum
Demetrova 1
10000 Zagreb
HR
地理涵蓋範圍

Croatia (Medvednica Mt.)

界定座標範圍 緯度南界 經度西界 [-90, -180], 緯度北界 經度東界 [90, 180]
分類群涵蓋範圍

Stoneflies

Order  Plecoptera (Stoneflies)
時間涵蓋範圍
起始日期 / 結束日期 2021-06-17 / 2022-03-04
計畫資料

Medvednica Nature Park, located near Croatia's capital and largest city, Zagreb, experiences significant urbanization, which strongly impacts the fauna within this ecosystem. Surprisingly, despite the area's diverse (micro)habitats, including the discovery of several rare species previously unknown in the Croatian fauna, comprehensive field research on any group of organisms, coupled with an integrative approach, has never been conducted in this region. In this study, we present the first insights into the genetic and morphological diversity of stoneflies, one of the most endangered groups of organisms overall. Phylogenetic reconstructions and species delineation methods revealed significant intraspecific haplotype variation in most examined species (e.g., Brachyptera seticornis, Isoperla grammatica, and Leuctra braueri), except for Leuctra prima. Additionally, our study has identified isolated populations that merit further in-depth investigation concerning morphology, genetics, and ecology. These findings contribute valuable knowledge to the conservation of stoneflies in this area.

計畫名稱 Utilizing public sequence databases to identify genetic diversity of stoneflies in Medvednica Nature Park
辨識碼 Dora Kermek, Nikola Pischiutta
經費來源 Croatian Science Foundation
研究區域描述 Medvednica Mt., Croatia
研究設計描述 Project provides the first insight into the genetic and morphological diversity of stoneflies in the Medvednica Nature Park, thereby contributing to the faunal knowledge of the area. Additionally, it establishes phylogenetic and phylogeographical relationships between populations of widely distributed species in Europe: Brachyptera seticornis, Isoperla grammatica, Leuctra braueri, and Leuctra prima. These relationships enhance understanding of diversification patterns and the biogeographic history of these selected stonefly species, emphasizing populations that warrant further detailed morphological, genetic, and ecological investigation.

參與計畫的人員:

取樣方法

Adults were collected using an entomological net while larvae were collected by hand under stones and branches.

研究範圍 Specimens were collected at eight sampling sites in Medvednica Nature Park.
品質控管 All collected individuals were preserved in ethanol. Morphological determination of adults was conducted using a ZEISS SteREO Discovery V20 stereo microscope along with various identification keys. Due to the lack of identification keys the larvae were not determined to the species level. Larval identification was carried out by comparing their sequences with sequences available in databases.

方法步驟描述:

  1. Total genomic DNA was isolated from the tissues of one to three legs of adults and larvae using the GenElute Mammalian Genomic DNA Miniprep Kit. Chromatograms were analyzed and edited in Geneious Prime. Misread nucleotides were manually corrected. The presence of stop codons in all sequences was checked using Mesquite. Poor quality sequences and those containing stop codons were excluded from further analyses. For phylogeographic analyses, we selected species with a broader distribution and a larger number of COI sequences available in the BOLD and GenBank databases.
收藏資料
蒐藏名稱 Collection Plecoptera Sivec&Hlebec
標本保存方法 Alcohol
管理單位 在...之間 488 和 512 vial
額外的元數據