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Kermek D, Pischiutta N, Hlebec D (2024): croplm. v1.2. Biodiversity Data Journal. Dataset/Occurrence. https://ipt.pensoft.net/resource?r=croplm&v=1.2

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Occurrence; Specimen; stoneflies; Medvednica Mt.; Croatia; biodiversity

Croatia (Medvednica Mt.)

Stoneflies

Medvednica Nature Park, located near Croatia's capital and largest city, Zagreb, experiences significant urbanization, which strongly impacts the fauna within this ecosystem. Surprisingly, despite the area's diverse (micro)habitats, including the discovery of several rare species previously unknown in the Croatian fauna, comprehensive field research on any group of organisms, coupled with an integrative approach, has never been conducted in this region. In this study, we present the first insights into the genetic and morphological diversity of stoneflies, one of the most endangered groups of organisms overall. Phylogenetic reconstructions and species delineation methods revealed significant intraspecific haplotype variation in most examined species (e.g., Brachyptera seticornis, Isoperla grammatica, and Leuctra braueri), except for Leuctra prima. Additionally, our study has identified isolated populations that merit further in-depth investigation concerning morphology, genetics, and ecology. These findings contribute valuable knowledge to the conservation of stoneflies in this area.

The personnel involved in the project:

Adults were collected using an entomological net while larvae were collected by hand under stones and branches.

Method step description:

1. Total genomic DNA was isolated from the tissues of one to three legs of adults and larvae using the GenElute Mammalian Genomic DNA Miniprep Kit. Chromatograms were analyzed and edited in Geneious Prime. Misread nucleotides were manually corrected. The presence of stop codons in all sequences was checked using Mesquite. Poor quality sequences and those containing stop codons were excluded from further analyses. For phylogeographic analyses, we selected species with a broader distribution and a larger number of COI sequences available in the BOLD and GenBank databases.